bio rad proteominer tm protein enrichment kit  (Bio-Rad)

Journal: Animals : an Open Access Journal from MDPI

Article Title: Proteomics Analysis of Pregnancy in Ewes under Heat Stress Conditions and Melatonin Administration

doi: 10.3390/ani14030400

Figure Lengend Snippet: The different proteins derived from a comparison between M3 and C3 after applying the ProteoMiner TM enrichment kit. The table provides their identities, biological process, protein coverage, molecular weight, theoretical pI and Mascot score as calculated with PDQuest 8.0 software.

Article Snippet: In total, 13 samples were analyzed in duplicates as follows: the samples M1 and C1 were pooled together (sample M1–C1), the samples M2, M3, M4 and M5 and C2, C3, C4 and C5 were assayed separately, and, additionally, the samples M3, C3, M5 and C5 were assayed after applying the ProteoMiner TM enrichment kit (Bio-Rad, Hercules, CA, USA).

Techniques: Derivative Assay, Comparison, Molecular Weight, Software, Activity Assay, Migration, Methylation, In Utero, DNA Methylation Assay, Cell Differentiation, Phospho-proteomics

Journal: Animals : an Open Access Journal from MDPI

Article Title: Proteomics Analysis of Pregnancy in Ewes under Heat Stress Conditions and Melatonin Administration

doi: 10.3390/ani14030400

Figure Lengend Snippet: The different proteins derived from a comparison between M5 and C5 after applying the ProteoMiner TM enrichment kit. The table provides their identities, biological process, protein coverage, molecular weight, theoretical pI and Mascot score as calculated with PDQuest 8.0 software.

Article Snippet: In total, 13 samples were analyzed in duplicates as follows: the samples M1 and C1 were pooled together (sample M1–C1), the samples M2, M3, M4 and M5 and C2, C3, C4 and C5 were assayed separately, and, additionally, the samples M3, C3, M5 and C5 were assayed after applying the ProteoMiner TM enrichment kit (Bio-Rad, Hercules, CA, USA).

Techniques: Derivative Assay, Comparison, Molecular Weight, Software, Cell Differentiation, Activation Assay, Immunopeptidomics, Membrane, Histone Deacetylase Assay, Gene Expression, Activity Assay, Phospho-proteomics, Methylation, In Utero, Transduction

Journal: Animals : an Open Access Journal from MDPI

Article Title: Proteomics Analysis of Pregnancy in Ewes under Heat Stress Conditions and Melatonin Administration

doi: 10.3390/ani14030400

Figure Lengend Snippet: Plasma samples from control ewes ( a ) and melatonin-treated ewes ( b ) and control ( c ) and melatonin ewes ( d ) after applying the ProteoMiner TM enrichment kit at parturition were analyzed via two-dimensional gel electrophoresis (2-DE). The gels were stained with Coomassie blue, and protein spots were excised and further analyzed via matrix-assisted laser desorption ionization time-of-flight mass spectrometry, as described in . In the gel images, each spot represents a protein. Comparison of protein profiles revealed proteins differentially expressed between the two groups analyzed (arrows). Protein accession numbers used are presented in and .

Article Snippet: In total, 13 samples were analyzed in duplicates as follows: the samples M1 and C1 were pooled together (sample M1–C1), the samples M2, M3, M4 and M5 and C2, C3, C4 and C5 were assayed separately, and, additionally, the samples M3, C3, M5 and C5 were assayed after applying the ProteoMiner TM enrichment kit (Bio-Rad, Hercules, CA, USA).

Techniques: Clinical Proteomics, Control, Two-Dimensional Gel Electrophoresis, Electrophoresis, Staining, Mass Spectrometry, Comparison

Journal: Molecules

Article Title: Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples

doi: 10.3390/molecules26092762

Figure Lengend Snippet: List of proteins identified in royal jelly found in: CRJ—“crude” (non-enriched with ProteoMiner TM technique) water extracts of royal jelly; F1, F2, F3, F4—separate fractions obtained from ProteoMiner TM elution with 1 M sodium chloride, 20 mM HEPES, pH 7.4 (F1), 200 mM glycine, pH 2.4 (F2), 60% ethylene glycol in water (F3), and 33.3% 2-propanol, 16.7% acetonitrile (ACN), 0.1% trifluoroacetic acid (TFA) (F4); MW—molecular weight; pI—isoelectric point. Fractions, in which proteins were detected, marked with “x”.

Article Snippet: Therefore, we proposed the ProteoMiner TM (Bio-Rad, Hercules, CA, USA) kit for proteins purifying and concentration.

Techniques: Ubiquitin Proteomics, Binding Assay, Clinical Proteomics, Variant Assay

Journal: Molecules

Article Title: Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples

doi: 10.3390/molecules26092762

Figure Lengend Snippet: The number of proteins identified in unpurified samples, samples pre-treated with ProteoMiner TM , and both in unpurified and purified samples.

Article Snippet: Therefore, we proposed the ProteoMiner TM (Bio-Rad, Hercules, CA, USA) kit for proteins purifying and concentration.

Techniques: Purification

Journal: Molecules

Article Title: Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples

doi: 10.3390/molecules26092762

Figure Lengend Snippet: Classes of proteins identified ( A ) in total in all RJ “crude” and pre-treated; ( B ) only in crude (non-ProteoMiner TM pre-treated) RJ samples; ( C ) only in ProteoMiner TM pre-treated RJ samples.

Article Snippet: Therefore, we proposed the ProteoMiner TM (Bio-Rad, Hercules, CA, USA) kit for proteins purifying and concentration.

Techniques: